This is an early access version
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Department of Orthopedic and Traumatology, Faculty of Medicine, Universitas Brawijaya, RSUD Prof. Dr. Saiful Anwar Malang , Malang , Indonesia
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Department of Orthopedic and Traumatology, Faculty of Medicine, Universitas Brawijaya, RSUD Prof. Dr. Saiful Anwar Malang , Malang , Indonesia
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Department of Orthopedic and Traumatology, Faculty of Medicine, Universitas Brawijaya, RSUD Prof. Dr. Saiful Anwar Malang , Malang , Indonesia
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Department of Physics, Faculty of Mathematics and Natural Science, University of Brawijaya , Malang , Indonesia
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Department of Orthopedic and Traumatology, Faculty of Medicine, Universitas Brawijaya, RSUD Prof. Dr. Saiful Anwar Malang , Malang , Indonesia
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Department of Biochemistry and Biomolecular, Faculty of Medicine, University of Brawijaya , Malang , Indonesia
Department of Orthopedic and Traumatology, Faculty of Medicine, Universitas Airlangga, Rumah Sakit Dokter Soetomo , Surabaya , Indonesia
Aim Orthopedic implants must meet specific criteria, including mechanical strength, durability, and biocompatibility. This study compares the immune response of zirconia, polyether ether ketone (PEEK), and stainless-steel implants in vivo, focusing on lymphocyte and fibroblast infiltration as indicators of immune activation.
Methods A total of 27 New Zealand white rabbits were used, with nine animals in each group. Implants of zirconia, PEEK, or stainless steel were surgically placed in the thigh and observed for 4 weeks. Histological analysis measured lymphocyte and fibroblast infiltration at the implant site using a microscope at 400x magnification. Statistical analysis included the Kruskal-Wallis test for group comparisons, followed by Mann-Whitney and Bonferroni correction for pairwise comparisons.
Results The Kruskal-Wallis test showed significant differences in lymphocyte (p=0.002) and fibroblast (p=0.003) counts among the groups. Zirconia exhibited significantly lower lymphocyte (median=0.5) and fibroblast (median=1.0) infiltration compared to stainless steel (lymphocytes: median=3.0, fibroblasts: median=2.0), and PEEK (lymphocytes: median=2.0, fibroblasts: median=3.0). Bonferroni correction confirmed zirconia showed the least immune activation (p<0.0167).
Conclusion Zirconia offers superior biocompatibility with minimal immune response, making it an ideal material for orthopedic implants, particularly for patients with metal sensitivities. PEEK showed moderate immune activation but is helpful for non-load-bearing applications. Stainless Steel induced the highest immune response due to the release of metal ions and corrosion. Zirconia is the most biocompatible material tested, making it a promising choice for orthopedic implants.
Conceptualization, A.R.B.S., R.S.D. and E.M.; Formal Analysis, A.R.B.S.; Methodology, A.R.B.S. and P.S.; Project administration, A.R.B.S.; Writing – original draft, A.R.B.S., R.S.D., E.M. and S.P.S.; Resources, R.S.D., E.M. and S.P.S.; Supervision, R.S.D., E.M. and S.P.S.; Validation, P.S., H.S. and H.S.; Visualization, P.S., H.S. and H.S.; Writing – review & editing, P.S., H.S. and H.S.; Software, H.S. All authors have read and agreed to the published version of the manuscript.
No specific funding was received for this study.
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